leaf tissue grinder

Two micro-scale protocols for the isolation of DNA from ... Place 0.01–0.03 g of silica gel-dried young leaf tissue in a microcentrifuge tube with a sterile grinder. Snap freeze by suspending the tube in liquid nitrogen and grind to a fine powder. 2. Add 1 ml of HEPES buffer (2% β-mercaptoethanol, 0.2% PVP, 0.1 M HEPES pH 8). A fully automatable enzymatic method for DNA extraction ... Leaf tissue was used in most cases and some seeds were also tested as indicated. Protocols Leaf disks (5 mm in diameter) were incubated in 50 μl of digestion buffer (175 mM EDTA [pH 8.0], 100 mM sodium acetate [pH 4.6], 1% triton X100) and 5 μl of the enzymatic cocktail. Potato Transformation —BIO-PROTOCOL Cut approximately one-half to one square centimeter of leaf tissue to a 1.5 ml centrifuge tube. Add 200 μl TPS to each tube. Grind the tissue in TPS with a tissue grinder to obtain a uniform slurry. Incubate samples at 75 °C for 20 min. Centrifuge at 13,400 x g for 10 min.    Read More

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